ExplorerĮxplorer plug-in arranges folders for a developer to figure out and navigate easily across numerous source code files and folders.Įxplorer displays folders and files on the left corner of the text editor window in a hierarchical order so that you can locate files without much a hustle. No need to memorize the codes! Sounds funny but that is what snippet plug-in came in to serve. The snippet plug-in allows developers to add various code snippets used often to make their coding experience a hobby.Įxcuse your memory for other productivity. We conclude that the FlexStation is a useful tool for high throughput studies when examining the function of neurotransmitter receptors that result in either a change in Ca2+ concentration or membrane potential.Apart from being highly customizable, it supports many third-party plug-ins to help developers while coding web applications and desktop applications, the snippet plug-in being a fundamental member of the team. Modifying the constituents of the buffer, however, so that the change in membrane potential was reduced, yielded EC50 values that were similar to previously reported data. The results show that the pharmacological characteristics of the receptor obtained using the FlexStation is similar to those reported using other functional methods, although caution must be applied when using the membrane potential dye as large changes in membrane potential can yield inaccurate EC50s. ![]() ![]() Here we have used the FlexStation to examine the function of recombinant 5-HT3 receptors expressed in HEK293 cells using both these classes of dye. The 5-HT3 receptor is one such protein, as its activation results in a change in membrane potential due to the opening of a Ca2+-permeant, cation-selective channel it can therefore be studied using both Ca2+- and membrane potential-sensitive fluorescent dyes. The FlexStation is a 96 or 384 fluorescent plate reader with the capability of adding solutions during readings it therefore has the potential to provide high throughput analyses of the functional characteristics of neurotransmitter-gated ion channels that can be examined using changes in fluorescence. Data are transferred from the SoftMax Pro software to construct concentration-response relationships of TRPA1 activators and inhibitors. The effect of a putative TRPA1 antagonist was also examined. HEK293 cells transiently or stably expressing human TRPA1 channels, grown in 96-well plates, are loaded with a Ca2+-sensitive fluorescent dye, Fluo-4, and real-time fluorescence changes in these cells are measured before and during the application of a TRPA1 agonist using the FLEX mode of the FlexStation 3. In this video, we demonstrate the application of FlexStation 3 to study the pharmacological profile of the TRPA1 channel, a molecular sensor for numerous noxious stimuli. Most of the TRP channels are calcium permeable and induce calcium influx upon activation. Transient receptor potential (TRP) channels are a large family of nonselective cation channels that play important roles in many physiological and pathophysiological functions. For example, FlexStation 3 has been used to study the function of Ca2+-permeable ion channels and G-protein coupled receptors by measuring the changes of intracellular free Ca2+ levels. It has an integrated fluid transfer module equipped with a multi-channel pipetter and the machine reads one column at a time to monitor fluorescence changes of a variety of fluorescent reagents. It is ideal for medium- to high-throughput screens in academic settings. The Molecular Devices' FlexStation 3 is a benchtop multi-mode microplate reader capable of automated fluorescence measurement in multi-well plates.
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